See also

Ligases

New Enzymes for Your Research

Restriction Nucleases
More Enzymes
& Companion

Methyl Specific & Methyl Tolerating Restriction Endonucleases

Selective for (mA, 5mC) Methylation

Cat#	Acronym		DNA Strands	Pack Size units
E486	Bis I	5mC	G(5mC)↑NGC	200
			CGN↓(5mC)G

E534	Bls I	5mC	G(5mC)N↑GC	500
			(5mC)G↓N(5mC)G

E494	Gla I	5mC	G(5mC)↑G(5mC)	500
			(5mC)G↓(5mC)G

E520	Glu I		(5mC)G(5mC)↑NG(5mC)G	500
			G(5mC)GN↓(5mC)G(5mC)

E506	Pcs I		W(5mC)GNNNN↑NNN(5mC)GW	250
			WG(5mC)NNN↓NNNNG(5mC)W

E490	Mal I	mA	G(mA)↑TC	250
			CT↓(mA)G

Alternative Tools for Epigenomic Profiling

Sensitive to dcm or CpG Methylation

Cat #	Acronym		DNA Strands	Pack size units
E474	Ajn I	6mC	↑CCWGG GGWCC↓	1000
E026	BgI I	6mC	GCCNNNN↑NGGC CGGN↓NNNNCCG	5000
E052	Bst2U I	6mC	CC↑WGG GGW↓CC	5000
E222	Asp LE I	5mC, 6mC	GCG↑C C↓GCG	2500
E286	Bdo31 I		GGTCTC(N)1↑ CCAGAG(N)5↓	500

Insensitive to dcm or CpG Methylation

Cat#	Acronym		DNA Strands	Pack size units
E004	ACC65 I		G↑GTACC CCATG↓G	5000
E020	Apa I	5mC	↑CCWGG GGWCC↓	5000
E180	Acc B71		CCANNNN↑NTGG GGTN↓NNNNACC	1000
E012	AcI I	5mC	AA↑CGTT TTGC↓AA	1000
E308	Bst SC I	5mC	↑CCNGG GGNCC↓	500
E302	Msp20I		TGG↑CCA ACC↓GGT	500

Buffers_Cutting_Sites.pdf

More DNA ENZYMES
Genomic DNA; DNA Markers

Restriction Endonucleases

Enzyme
Prototype
Recognition sequence

Cat. No.
Package u.a
SE Buffer 100% ACTIV
SE Buffer 75-100% ACTIV

Aat II
Aat II
GACGT^C
E287
500
Y

C^TGCAG
E288
2500

Abs I
Abs I
CC^TCGAGG
E535
50
*
B
GGAGCT^CC
E536
250

Acc16 I
Mst I
TGC^GCA
E001
200
W
G
ACG^CGT
E002
1000

Acc36 I
BspM I
ACCTGC(N)4^
E289
100
Y

TGGACG(N)8^
E290
500

Acc65 I
(Kpn I)
G^GTACC
E003
1000
W
O
CCATG^G
E004
5000

AccB1 I
HgiC I
G^GYRCC
E163
500
K
W
CCRYG^G
E164
2500

AccB7 I
PflM I
CCANNNN^NTGG
E179
200
G

GGTN^NNNNACC
E180
1000

AccBS I
BsrB I
GAG^CGG
E007
1000
Y
G,B
CTC^GCC
E008
5000

Acl I
Acl I
AA^CGTT
E011
200
Y

TTGC^AA
E012
1000

AclW I
Bin I
GGATC(N)4^
E211
100
Y
B
CCTAG(N)5^
E212
500

Aco I
Cfr I
Y^GGCCR
E499
100
G

RCCGG^Y
E500
500

Acs I
Apo I
R^AATTY
E013
500
W

YTTAA^R
E014
2500

Acu I
Eco57 I
CTGAAG(N)16^
E451
50
Y+SAM
W
GACTTC(N)14^
E452
250

Afe I
Eco47 III

E213
200
Y
W
AGC^GCT
E214
1000

TCG^CGA
E214X
1000

Ags I
Ags I
TTS^AA
E573
200
Y
B
AA^STT
E574
1000

Ahl I
Spe I
A^CTAGT
E173
1000
B
G,Y
TGATC^A
E174
5000

Ajn I
EcoR II
^CCWGG
E473
200
Y

GGWCC^
E474
1000

Alu I
Alu I
AG^CT
E015
200
Y
B,G
TC^GA
E016
1000

AluB I
Alu I
AG^CT
E549
200
B
G
TC^GA
E550
1000

Ama87 I
Ava I
C^YCGRG
E017
1000
W
O
GRGCY^C
E018
5000

Apa I
Apa I
GGGCC^C
E019
1000
Y

C^CCGGG
E020
5000

Ars I
Ars I
^(N)8GAC(N)6TTYG(N)11^
E575
50
Y

^(N)13CTG(N)6AARC(N)6^
E576
250

AsiG I
Age I
A^CCGGT
E235
100
O
W
TGGCC^A
E236
500

AspA2 I
Avr II
C^CTAGG
E245
500
W
O,Y
GGATC^C
E246
2500

AspLE I
Hha I
GCG^C
E221
500
O
G
C^GCG
E222
2500

AspS9 I
Sau96 I
G^GNCC
E117
1000
W
O
CCNG^G
E118
5000

AsuC2 I
Cau II
CC^SGG
E257
2000
Y
B
GGS^CC
E258
10000

AsuHP I
Hph I
GGTGA(N)8^
E231
200
O

CCACT(N)7^
E232
1000

AsuNH I
Nhe I
G^CTAGC
E063
500
Y
B
CGATC^G
E064
2500

BamH I
BamH I

E021
4000
G
O,W
G^GATCC
E022
20000

CCTAG^G
E021X
4000

E022X
20000

Bar I
Bar I
^(N)7GAAG(N)6TAC(N)12^
E547
100
2K

^(N)12CTTC(N)6ATG(N)7^
E548
500

Bbv12 I
HgiA I
GWGCW^C
E023
200
O
W
C^WCGWG
E024
1000

Bgl I
Bgl I
GCCNNNN^NGGC
E025
1000
2W

CGGN^NNNNCCG
E026
5000

Bgl II
Bgl II
A^GATCT
E027
1000
O

TCTAG^A
E028
5000

Bis I
Bis I
G(5mC)^NGC
E485
40
*
W
CGN^(5mC)G
E486
200

Bls I
Bls I
G(5mC)N^GC
E533
100
W

(5mC)G^N(5mC)G
E534
500

Bme18 I
Ava II
G^GWCC
E029
1000
O
W
CCWG^G
E030
5000

Bmt I
Nhe I
GCTAG^C
E457
1000
W
Y
C^GATCG
E458
5000

Bmu I
Bfi I
ACTGGG(N)5^
E487
100
Y
B,G
TGACCC(N)4^
E488
500

Bpm I
Gsu I
CTGGAG(N)16^
E467
50
W
O
GACCTC(N)14^
E468
250

Bpu10 I
Bpu10 I
CC^TNAGC
E149
200
K

GGANT^CG
E150
1000

Bpu14 I
Asu II
TT^CGAA
E033
1000
G
Y
AAGC^TT
E034
5000

Bsa29 I
Cla I
AT^CGAT
E205
1000
G
Y
TAGC^TA
E206
5000

Bsc4 I
BsiY I
CCNNNNN^NNGG
E219
500
W
B,G
GGNN^NNNNNCC
E220
2500

* means individual buffer; color denotes buffers matching activity range for given set of enzymes. Buffer Components see here

Bse1 I through MroX I

Enzyme	Prototype	Recognition sequence	Cat.No.	Package a.u.	SE Buffer 100% Activ	SE Buffer 75-100% Activ
Bse1 I	Bsr I	ACTGGN^	E035	500	Y	B,G
Bse1 I	Bsr I	TGAC^CN	E036	2500
Bse118 I	Cfr10 I	R^CCGGY	E039	200	O	W
Bse118 I	Cfr10 I	YGGCC^R	E040	1000
Bse21 I	Sau I	CC^TNAGG	E037	500	Y
Bse21 I	Sau I	GGANT^CC	E038	2500
Bse3D I	BsrD I	GCAATGNN^	E253	200	G	Y
Bse3D I	BsrD I	CGTTAC^NN	E254	1000
Bse8 I	BsaB I	GATNN^NNATC	E147	1000	G	O,W
Bse8 I	BsaB I	CTANN^NNTAG	E148	5000
BseP I	BseP I	G^CGCGC	E181	200	G	O
BseP I	BseP I	CGCGC^G	E182	1000
BseX3 I	Xma III	C^GGCCG	E263	200	O	G,W
BseX3 I	Xma III	GCCGG^C	E264	1000
BslF I	Fin I	GGGAC(N)10^	E479	100	Y
BslF I	Fin I	CCCTG(N)14^	E480	500
		GGGAC(N)11^
		CCCTG(N)15^
Bso31I	Eco31I	GGTCTCN^	E285	100	O	W
Bso31I	Eco31I	CCAGAG(N)5^	E286	500
Bsp13 I	BspM II	T^CCGGA	E183	500	2K	O
Bsp13 I	BspM II	AGGCC^T	E184	2500
Bsp1720 I	Esp I	GC^TNAGC	E185	100	G	Y
Bsp1720 I	Esp I	CGANT^CG	E186	500
Bsp19 I	Nco I	C^CATGG	E047	500	2W	W
Bsp19 I	Nco I	GGTAC^C	E048	2500
BspAC I	Aci I	C^CGC	E501	200	O	W
BspAC I	Aci I	GGC^G	E502	1000
BspFN I	FnuD II	CG^CG	E557	500	Y	G,O
BspFN I	FnuD II	GC^GC	E558	2500
BssEC I	Sec I	C^CNNGG	E273	200	Y	W
BssEC I	Sec I	GGNNC^C	E274	1000
BssNA I	Sna I	GTA^TAC	E261	1000	W	O,Y
BssNA I	Sna I	CAT^ATG	E262	5000
BssT1 I	Sty I	C^CWWGG	E207	1000	2K	W
BssT1 I	Sty I	GGWWC^C	E208	5000
Bst2B I	Bsi I	C^TCGTG	E043	200	Y	B
Bst2B I	Bsi I	GAGCA^C	E044	1000
Bst2U I	BstN I	CC^WGG	E051	1000	G	B
Bst2U I	BstN I	GGW^CC	E052	5000
Bst4C I	Tsp4C I	ACN^GT	E265	200	Y	B,G
Bst4C I	Tsp4C I	TG^NCA	E266	1000
Bst6 I	Ksp632 I	CTCTTCN^	E239	200	Y	B,G,W
Bst6 I	Ksp632 I	GAGAAG(N)4^	E240	1000
BstAC I	Acy I	GR^CGYC	E093	500	W	B,G,Y
BstAC I	Acy I	CYGC^RG	E094	2500
BstAF I	Afl II	C^TTAAG	E135	1000	W	O
BstAF I	Afl II	GAATT^C	E136	5000
BstAP I	(ApaB I)	GCANNNN^NTGC	E259	200	W	O
BstAP I	(ApaB I)	CGTN^NNNNACG	E260	1000
BstAU I	Bsp1407 I	T^GTACA	E267	500	W
BstAU I	Bsp1407 I	ACATG^T	E268	2500
BstBA I	BsaA I	YAC^GTR	E237	500	W	O
BstBA I	BsaA I	RTG^CAY	E238	2500
BstC8 I	Cac8 I	GCN^NGC	E305	200	Y	W
BstC8 I	Cac8 I	CGN^NCG	E306	1000
BstDE I	Dde I	C^TNAG	E227	500	G	B
BstDE I	Dde I	GANT^C	E228	2500
BstDS I	Dsa I	C^CRYGG	E083	1000	Y	G
BstDS I	Dsa I	GGYRC^C	E084	5000
BstEN I	EcoN I	CCTNN^NNNAGG	E103	200	Y
BstEN I	EcoN I	GGANNN^NNTCC	E104	1000
BstF5 I	(Fok I)	GGATGNN^	E031	500	Y	B
BstF5 I	(Fok I)	CCTAC^NN	E032	2500
BstFN I	FnuD II	CG^CG	E283	300	Y	B
BstFN I	FnuD II	GC^GC	E284	1500
BstH2 I	Hae II	RGCGC^Y	E171	500	Y
BstH2 I	Hae II	Y^CGCGR	E172	2500
BstHH I	Hha I	GCG^C	E143	2000	Y	B
BstHH I	Hha I	C^GCG	E144	10000
BstKT I	Mbo I	GAT^C	E151	200	W	O
BstKT I	Mbo I	C^TAG	E152	1000
BstMA I	BsmA I	GTCTCN^	E291	2000	W	Y
BstMA I	BsmA I	CAGAG(N)5^	E292	10000
BstMB I	Mbo I	^GATC	E119	200	O	W
BstMB I	Mbo I	CTAG^	E120	1000
BstMC I	Mcr I	CGRY^CG	E071	500	B	G
BstMC I	Mcr I	GC^YRGC	E072	2500
BstMW I	Mwo I	GCNNNNN^NNGC	E459	500	Y
BstMW I	Mwo I	CGNN^NNNNNCG	E460	2500
BstNS I	Nsp I	RCATG^Y	E251	200	B	Y
BstNS I	Nsp I	Y^GTACR	E252	1000
BstPA I	PshA I	GACNN^NNGTC	E299	1000	Y
BstPA I	PshA I	CTGNN^NNCAG	E300	5000
BstSC I	ScrF I	^CCNGG	E307	100	Y
BstSC I	ScrF I	GGNCC^	E308	500
BstSF I	Sfe I	C^TRYAG	E197	200	O	B
BstSF I	Sfe I	GAYRT^C	E198	1000
BstSL I	BseS I	GKGCM^C	E561	200	G	W,Y
BstSL I	BseS I	C^MCGKG	E562	1000
BstSN I	SnaB I	TAC^GTA	E065	200	B
BstSN I	SnaB I	ATG^CAT	E066	1000
BstV1 I	Bbv I	GCAGC(N)8^	E303	100	G	B,O,W
BstV1 I	Bbv I	CGTCG(N)12^	E304	500
BstV2 I	Bbv II	GAAGAC(N)2^	E297	200	Y	B,G
BstV2 I	Bbv II	CTTCTG(N)6^	E298	1000
BstX I	BstX I	CCANNNNN^NTGG	E465	200	O	W
BstX I	BstX I	GGTN^NNNNNACC	E466	1000
BstX2 I	Xho II	R^GATCY	E229	500	G	B
BstX2 I	Xho II	YCTAG^R	E230	2500
BsuR I	Hae III	GG^CC	E053	1000	G	B
BsuR I	Hae III	CC^GG	E054	5000
Btr I	Btr I	CAC^GTC	E277	100	O	B,G,W,Y
Btr I	Btr I	GTG^CAG	E278	500
Cci I	BspH I	T^CATGA	E565	1000	W	Y
Cci I	BspH I	AGTAC^T	E566	5000
CciN I	Not I	GC^GGCCGC	E203	200	Y	O,W
CciN I	Not I	CGCCGG^CG	E204	1000
Dra I	Aha III	TTT^AAA	E055	1000	G	B,W,Y
Dra I	Aha III	AAA^TTT	E056	5000
Dra III	Dra III	CACNNN^GTG	E309	500	2K	O,W
Dra III	Dra III	GTG^NNNCAC	E310	2500
Dri I	Eam1105 I	GACNNN^NNGTC	E193	200	Y	B,G
Dri I	Eam1105 I	CTGNN^NNNCAG	E194	1000
DseD I	Drd I	GACNNNN^NNGTC	E241	500	Y	B,G
DseD I	Drd I	CTGNN^NNNNCAG	E242	2500
EcoICR I	Sac I	GAG^CTC	E469	200	G	B,Y
EcoICR I	Sac I	CTC^GAG	E470	1000
EcoR I	EcoR I		E057	5000	*	G,O,W
		G^AATTC	E058	25000	*
		CTTAA^G	E057X	5000	*
			E058X	25000	*
EcoR V	EcoR V	GAT^ATC	E059	2000	W
EcoR V	EcoR V	CTA^TAG	E060	10000
Ege I	Nar I	GGC^GCC	E243	200	B	G,Y
Ege I	Nar I	CCG^CGG	E244	1000
Erh I	Sty I	C^CWWGG	E061	1000	2W	W
Erh I	Sty I	GGWWC^C	E062	5000
Fae I	Nla III	CATG^	E495	50	Y
Fae I	Nla III	^GTAC	E496	250
Fai I	Fai I	YA^TR	E551	50	B
Fai I	Fai I	RT^AY	E552	250
Fal I	Fal I	^(N)8AAGNNNNNCTT(N)13^	E153	100	W+SAM	O
Fal I	Fal I	^(N)13TTCNNNNNGAA(N)8^	E154	500
Fat I	Nla III	^CATG	E155	50	G
Fat I	Nla III	GTAC^	E156	250
Fau I	Fau I	CCCGC(N)4^	E209	100	B
Fau I	Fau I	GGGCG(N)6^	E210	500
FauND I	Nde I	CA^TATG	E009	500	Y	G
FauND I	Nde I	GTAT^AC	E010	2500
Fbl I	Acc I	GT^MKAC	E271	100	Y	G
Fbl I	Acc I	CAKM^TG	E272	500
Fok I	Fok I	GGATG(N)9^	E247	100	Y
Fok I	Fok I	CCTAC(N)13^	E248	500
FriO I	Ban II	GRGCY^C	E157	1000	Y	B,G
FriO I	Ban II	C^YCGRG	E158	5000
Fsp4H I	Fnu4H I	GC^NGC	E095	100	Y	G
Fsp4H I	Fnu4H I	CGN^CG	E096	500
Gla I	Gla I	G(5mC)^G(5mC)	E493	100	*	B,G,O,W,Y
Gla I	Gla I	(5mC)G^(5mC)G	E494	500
Glu I	Glu I	(5mC)G(5mC)^NG(5mC)G	E519	100	*	B,G,O,W,Y
Glu I	Glu I	G(5mC)GN^(5mC)G(5mC)	E520	500
Gsa I	BseY I	CCCAG^C	E563	1000	W	O,Y
Gsa I	BseY I	G^GGTCG	E564	5000
Hae III	Hae III		E067	2000	G	B
		GG^CC	E068	10000
		CC^GG	E068X	10000
Hga I	Hga I	GACGC(N)5^	E461	50	B	G
Hga I	Hga I	CTGCG(N)10^	E462	250
Hind II	Hind II	GTY^RAC	E201	1000	G	B,Y
Hind II	Hind II	CAR^YTG	E202	5000
Hind III	Hind III		E073	5000	W
		A^AGCTT	E074	25000
		TTCGA^A	E073X	5000
			E074X	25000
Hinf I	Hinf I		E075	1000	O	G,W,Y
		G^ANTC	E076	5000
		CTNA^G	E075X	1000
			E076X	5000
Hpa I	Hpa I	GTT^AAC	E077	500	Y
Hpa I	Hpa I	CAA^TTG	E078	2500
Hpa II	Hpa II	C^CGG	E161	500	B
Hpa II	Hpa II	GGC^C	E162	2500
HspA I	(Hha I)	G^CGC	E069	1000	Y
HspA I	(Hha I)	CGC^G	E070	5000
Kpn I	Kpn I		E079	2000	B	Y
		GGTAC^C	E080	10000
		C^CATGG	E079X	2000
			E080X	10000
Ksp22 I	Bcl I	T^GATCA	E081	1000	2K
Ksp22 I	Bcl I	ACTAG^T	E082	5000
Kzo9 I	Mbo I	^GATC	E187	200	G
Kzo9 I	Mbo I	CTAG^	E188	1000
Mab I	SexA I	A^CCWGGT	E121	200	W	O
Mab I	SexA I	TGGWCC^A	E122	1000
Mal I	Dpn I	G(mA)^TC	E489	50	*	W
Mal I	Dpn I	CT^(mA)G	E490	250
Mbo II	Mbo II	GAAGA(N)8^	E471	200	Y	B,G
Mbo II	Mbo II	CTTCT(N)7^	E472	1000
Mhl I	Sdu I	GDGCH^C	E049	500	W	O
Mhl I	Sdu I	C^HCGDG	E050	2500
Mlu I	Mlu I	A^CGCGT	E085	1000	O
Mlu I	Mlu I	TGCGC^A	E086	5000
Mly113 I	Nar I	GG^CGCC	E189	200	B
Mly113 I	Nar I	CCGC^GG	E190	1000
Mnl I	Mnl I	CCTC(N)7^	E481	500	G	B,Y
Mnl I	Mnl I	GGAG(N)6^	E482	2500
MroN I	(Nae I)	G^CCGGC	E087	500	B
MroN I	(Nae I)	CGGCC^G	E088	2500
MroX I	Xmn I	GAANN^NNTTC	E249	200	W
MroX I	Xmn I	CTTNN^NNAAG	E250	1000		Top

* means individual buffer; color denotes buffers matching activity range for given set of enzymes. Buffer Components see here

Buffer Selection: Compatibility see here

Enzyme	Prototype	Recognition sequence	Cat. No.	Package u.a	SE Buffer 100% ACTIV	SE Buffer 75-100% ACTIV
Msp I	Hpa II	C^CGG	E091	1000	B	G,W,Y
Msp I	Hpa II	GGC^C	E092	5000
Msp20 I	Bal I	TGG^CCA	E301	100	W
Msp20 I	Bal I	ACC^GGT	E302	500
MspA1 I	NspB II	CMG^CKG	E191	500	Y	G
MspA1 I	NspB II	GKC^GMC	E192	2500
MspR9 I	ScrF I	CC^NGG	E175	500	O	Y
MspR9 I	ScrF I	GGN^CC	E176	2500
Nru I	Nru I	TCG^CGA	E099	500	W	O
Nru I	Nru I	AGC^GCT	E100	2500
PalA I	Asc I	GG^CGCGCC	E483	100	Y
PalA I	Asc I	CCGCGC^GG	E484	500
Pce I	Stu I	AGG^CCT	E105	1000	Y	B,G
Pce I	Stu I	TCC^GGA	E106	5000
Pci I	BspLU11 I	A^CATGT	E275	200	O	G,W
Pci I	BspLU11 I	TGTAC^A	E276	1000
PciS I	Sap I	GCTCTTCN^	E497	50	B	Y
PciS I	Sap I	CGAGAAG(N)₄^	E498	250
Pcs I	Pcs I	W(5mC)GNNNN^NNN(5mC)GW	E505	50	*
Pcs I	Pcs I	WG(5mC)NNN^NNNNG(5mC)W	E506	250
Pct I	Bsm I	GAATGCN^	E045	1000	O	W
Pct I	Bsm I	CTTAC^GN	E046	5000
Ple19 I	Pvu I	CGAT^CG	E195	100	Y	B,G
Ple19 I	Pvu I	GC^TAGC	E196	500
Pps I	Ple I	GAGTC(N)₄^	E269	25	Y
Pps I	Ple I	CTCAG(N)₅^	E270	125
Psi I	Psi I	TTA^TAA	E279	100	B	Y
Psi I	Psi I	AAT^ATT	E280	500
Psp124B I	Sac I	GAGCT^C	E107	1000	G	B,Y
Psp124B I	Sac I	C^TCGAG	E108	5000
Psp6 I	EcoR II	^CCWGG	E453	100	B	Y
Psp6 I	EcoR II	GGWCC^	E454	500
PspC I	PmaC I	CAC^GTG	E475	2000	B
PspC I	PmaC I	GTG^CAC	E476	10000
PspE I	BstE II	G^GTNACC	E169	2000	B
PspE I	BstE II	CCANTG^G	E170	10000
PspL I	Spl I	C^GTACG	E223	300	Y	B,G
PspL I	Spl I	GCATG^C	E224	1500
PspN4 I	Nla IV	GGN^NCC	E089	1000	Y
PspN4 I	Nla IV	CCN^NGG	E090	5000
PspOM I	(Apa I)	G^GGCCC	E215	1500	Y	B
PspOM I	(Apa I)	CCCGG^G	E216	7500
PspPP I	PpuM I	RG^GWCCY	E255	100	Y
PspPP I	PpuM I	YCCWG^GR	E256	500
PspX I	PspX I		E477	200	Y	W
		VC^TCGAGB	E478	1000
		BGAGCT^CV	E478X	1000
Psr I	Psr I	^(N)₇GAACNNNNNNTAC(N)₁₂^	E131	100	Y
Psr I	Psr I	^(N)₁₂CTTGNNNNNNATG(N)₇^	E132	500
Pst I	Pst I		E109	4000	O
			E110	20000
		CTGCA^G	E109X	4000
		G^ACGTC	E110X	20000
Pvu II	Pvu II	CAG^CTG	E111	2000	G
Pvu II	Pvu II	GTC^GAC	E112	10000
Rga I	Sgf I	GCGAT^CGC	E491	200	Y	B
Rga I	Sgf I	CGC^TAGCG	E492	1000
Rig I	Fse I	GGCCGG^CC	E529	100	*	B
Rig I	Fse I	CC^GGCCGG	E530	500
Rsa I	Rsa I	GT^AC	E113	1000	B	Y
Rsa I	Rsa I	CA^TG	E114	5000
RsaN I	Rsa I	G^TAC	E555	200	B	Y
RsaN I	Rsa I	CAT^G	E556	1000
Rsr2 I	Rsr II	CG^GWCCG	E281	1000	Y	G
Rsr2 I	Rsr II	GCCWG^GC	E282	5000
Sal I	Sal I	G^TCGAC	E115	2000	O
Sal I	Sal I	CAGCT^G	E116	10000
Sbf I	Sse8387 I	CCTGCA^GG	E101	200	Y	B
Sbf I	Sse8387 I	GG^ACGTCC	E102	1000
Set I	Set I		E537	200	Y	O,W
		ASST^	E538	1000
		^TSSA	E538X	1000
SfaN I	SfaN I	GCATC(N)₅^	E165	500	O	W
SfaN I	SfaN I	CGTAG(N)₉^	E166	2500
Sfi I	Sfi I		E123	1000	G	B
			E124	5000
		GGCCNNNN^NGGCC	E123X	1000
		CCGGN^NNNNCCGG	E124X	5000
Sfr274 I	Xho I	C^TCGAG	E125	2000	B	G,Y
Sfr274 I	Xho I	GAGCT^C	E126	10000
Sfr303 I	Sac II	CCGC^GG	E127	1000	B	Y
Sfr303 I	Sac II	GG^CGCC	E128	5000
Sma I	Sma I	CCC^GGG	E177	2000	Y
Sma I	Sma I	GGG^CCC	E178	10000
Smi I	Swa I	ATTT^AAAT	E225	1000	O	W
Smi I	Swa I	TAAA^TTTA	E226	5000
SmiM I	Msl I	CAYNN^NNRTG	E293	200	W	O
SmiM I	Msl I	GTRNN^NNYAC	E294	1000
Sph I	Sph I	GCATG^C	E129	500	G	O,W
Sph I	Sph I	C^GTACG	E130	2500
Sse9 I	Tsp509 I	^AATT	E217	500	B	G,Y
Sse9 I	Tsp509 I	TTAA^	E218	2500
Ssp I	Ssp I	AAT^ATT	E041	500	K	B,Y
Ssp I	Ssp I	TTA^TAA	E042	2500
Taq I	Taq I	T^CGA	E133	2000	Y	G,O
Taq I	Taq I	AGC^T	E134	10000
Tru9 I	Mse I	T^TAA	E199	500	W	B
Tru9 I	Mse I	AAT^T	E200	2500
Tth111 I	Tth111 I	GACN^NNGTC	E097	400	Y	B
Tth111 I	Tth111 I	CTGNN^NCAG	E098	2000
Vne I	ApaL I	G^TGCAC	E137	1000	O
Vne I	ApaL I	CACGT^G	E138	5000
Vsp I	Vsp I	AT^TAAT	E139	1000	W
Vsp I	Vsp I	TAAT^TA	E140	5000
Xba I	Xba I	T^CTAGA	E141	2000	O	B,G
		AGATC^T	E142	10000
			E141X	2000
			E142X	10000
Xma I	Xma I	C^CCGGG	E233	300	Y	B
Xma I	Xma I	GGGCC^C	E234	1500
Zra I	(Aat II)	GAC^GTC	E463	200	B	Y
Zra I	(Aat II)	CTG^CAG	E464	1000
Zrm I	Sca I	AGT^ACT	E005	600	Y
Zrm I	Sca I	TCA^TGA	E006	3000
Zsp2 I	Nsi I	ATGCA^T	E145	1000	B
Zsp2 I	Nsi I	T^ACGTA	E146	5000


NICKASE
N.Bst9 I		GAGTCNNNN^NN	E401	100	*	G
		CTCAGNNNNNN	E402	500	Top

buffer components see here

DNA Enzymes

DNA METHYLTRANSFERASES		Cat.No	Package u.a.
M3.BstF5 I 5mC	GG(mA)TG	E284	1500
M.Fsp4H I	G(5mC)NGC	E096	500
M.HspA I 5mC 5mC	G(5mC)GC	E070	5000

Enzyme		Cat.No	Package u.a.
Alkaline Phosphatase		E327	100
		E328	500
Thermolabile Alkaline Phosphatase		E365	200
		E366	1000
T4 Polynucleotidekinase		E311	500
		E312	2500
T4 DNA Ligase		E319	8000
		E320	40000
Concentrated T4 DNA Ligase, (2 000 000 units/ml)		E329	40000
		E330	200000
T4 RNA Ligase		E349	1000
		E350	5000
DNA Polymerase I E.coli, (Klenow fragment)		E325	200
		E326	1000
Taq DNA Polymerase with AS Buffer		E337	200
		E338	1000
Taq DNA Polymerase with Standard Taq Buffer		E331	200
(no Mg ²⁺ E341 /E342)		E332	1000
Taq SE DNA Polymerase		E313	200
Taq SE DNA Polymerase		E314	1000
Hot Start Taq DNA Polymerase		E351	200
		E352	1000
Pfu DNA Polymerase		E353	200
		E354	1000
T4 DNA Polymerase		E339	200
		E340	1000
M-MulV Reverse Transcriptase		E317	5000
		E318	25000
T7 RNA Polymerase		E355	5000
		E356	25000
Exonuclease II		E345	4000
		E346	20000
Uracil-DNA Glycosylase(UDG)		E335	200
		E336	1000
Tte-inorganic pyrophosphatase		E333	20
		E334	100
Tli-inorganic pyrophosphatase		E315	100
		E316	500
Endonuclease I		E323	4000
		E324	20000
DNA PREPARATIONS		Cat. No	Package u.a.
DNA phage T7		D02	500
			50
DNA phage lambda (dam-, dcm-)		D10	500
DNA phage lambda		D11	500

DNA pBR322		D03	50
		D04	250
DNA pUC19		D05	50
		D06	250
pHspAI2/GsaI DNA		D09	10

HUMAN GENOMIC DNA

Preparation		Cat. No:	Package, μg
Genomic DNA HeLa		D07	10
Genomic DNA Jurkat		D08	10
Genomic DNA Raji		D13	10
Genomic DNA U-937		D12	10
Genomic DNA L-68		D14	10

DNA MARKERS

Preparation		Cat.No.	Package, μg
Lambda DNA/HindIII		M01	100
		M02	500
Lambda DNA/Bme18I(AvaII)		M03	100
		M04	500
Lambda DNA/BssT1I(StyI)		M05	100
		M06	500
Lambda DNA/BglI		M17	100
		M18	500
Lambda DNA/BstEII		M09	100
		M10	500
pUC19/MspI		M07	50
		M08	250
pUC19/Kzo9I		M13	50
		M14	250
pBR322/AluI		M19	50
		M20	250
pBR322/BsuRI		M21	50
		M22	250
1 Kb DNA Ladder		M11	50
		M12	250
100 bp DNA Ladder		M15	50
		M16	250
100 bp+50 bp DNA Ladder		M33	50
		M34	250
100 bp+1.5 Kb DNA Ladder		M23	50
		M24	250
100 bp + 1.5 Kb + 3 Kb DNA Ladder		M27	50
		M28	250
100 bp + 2 Kb + 3 Kb DNA Ladder		M25	50
		M26	250
50 Kb DNA Ladder		M29	50
		M30	250

Enzyme	Prototype	Recognition sequence	Cat. No.	Package u.a	SE Buffer 100% ACTIV	SE Buffer 75-100% ACTIV
Enzyme	Prototype	Recognition sequence	Cat. No.		SE Buffer 100% ACTIV	SE Buffer 75-100% ACTIV
Aat II	Aat II	GACGT^C	E287	500	Y
Aat II	Aat II	C^TGCAG	E288	2500
Abs I	Abs I	CC^TCGAGG	E535	50	*	B
Abs I	Abs I	GGAGCT^CC	E536	250
Acc16 I	Mst I	TGC^GCA	E001	200	W	G
Acc16 I	Mst I	ACG^CGT	E002	1000
Acc36 I	BspM I	ACCTGC(N)4^	E289	100	Y
Acc36 I	BspM I	TGGACG(N)8^	E290	500
Acc65 I	(Kpn I)	G^GTACC	E003	1000	W	O
Acc65 I	(Kpn I)	CCATG^G	E004	5000
AccB1 I	HgiC I	G^GYRCC	E163	500	K	W
AccB1 I	HgiC I	CCRYG^G	E164	2500
AccB7 I	PflM I	CCANNNN^NTGG	E179	200	G
AccB7 I	PflM I	GGTN^NNNNACC	E180	1000
AccBS I	BsrB I	GAG^CGG	E007	1000	Y	G,B
AccBS I	BsrB I	CTC^GCC	E008	5000
Acl I	Acl I	AA^CGTT	E011	200	Y
Acl I	Acl I	TTGC^AA	E012	1000
AclW I	Bin I	GGATC(N)4^	E211	100	Y	B
AclW I	Bin I	CCTAG(N)5^	E212	500
Aco I	Cfr I	Y^GGCCR	E499	100	G
Aco I	Cfr I	RCCGG^Y	E500	500
Acs I	Apo I	R^AATTY	E013	500	W
Acs I	Apo I	YTTAA^R	E014	2500
Acu I	Eco57 I	CTGAAG(N)16^	E451	50	Y+SAM	W
Acu I	Eco57 I	GACTTC(N)14^	E452	250
Afe I	Eco47 III		E213	200	Y	W
		AGC^GCT	E214	1000
		TCG^CGA	E214X	1000
Ags I	Ags I	TTS^AA	E573	200	Y	B
Ags I	Ags I	AA^STT	E574	1000
Ahl I	Spe I	A^CTAGT	E173	1000	B	G,Y
Ahl I	Spe I	TGATC^A	E174	5000
Ajn I	EcoR II	^CCWGG	E473	200	Y
Ajn I	EcoR II	GGWCC^	E474	1000
Alu I	Alu I	AG^CT	E015	200	Y	B,G
Alu I	Alu I	TC^GA	E016	1000
AluB I	Alu I	AG^CT	E549	200	B	G
AluB I	Alu I	TC^GA	E550	1000
Ama87 I	Ava I	C^YCGRG	E017	1000	W	O
Ama87 I	Ava I	GRGCY^C	E018	5000
Apa I	Apa I	GGGCC^C	E019	1000	Y
Apa I	Apa I	C^CCGGG	E020	5000
Ars I	Ars I	^(N)8GAC(N)6TTYG(N)11^	E575	50	Y
Ars I	Ars I	^(N)13CTG(N)6AARC(N)6^	E576	250
AsiG I	Age I	A^CCGGT	E235	100	O	W
AsiG I	Age I	TGGCC^A	E236	500
AspA2 I	Avr II	C^CTAGG	E245	500	W	O,Y
AspA2 I	Avr II	GGATC^C	E246	2500
AspLE I	Hha I	GCG^C	E221	500	O	G
AspLE I	Hha I	C^GCG	E222	2500
AspS9 I	Sau96 I	G^GNCC	E117	1000	W	O
AspS9 I	Sau96 I	CCNG^G	E118	5000
AsuC2 I	Cau II	CC^SGG	E257	2000	Y	B
AsuC2 I	Cau II	GGS^CC	E258	10000
AsuHP I	Hph I	GGTGA(N)8^	E231	200	O
AsuHP I	Hph I	CCACT(N)7^	E232	1000
AsuNH I	Nhe I	G^CTAGC	E063	500	Y	B
AsuNH I	Nhe I	CGATC^G	E064	2500
BamH I	BamH I		E021	4000	G	O,W
		G^GATCC	E022	20000
		CCTAG^G	E021X	4000
			E022X	20000
Bar I	Bar I	^(N)7GAAG(N)6TAC(N)12^	E547	100	2K
Bar I	Bar I	^(N)12CTTC(N)6ATG(N)7^	E548	500
Bbv12 I	HgiA I	GWGCW^C	E023	200	O	W
Bbv12 I	HgiA I	C^WCGWG	E024	1000
Bgl I	Bgl I	GCCNNNN^NGGC	E025	1000	2W
Bgl I	Bgl I	CGGN^NNNNCCG	E026	5000
Bgl II	Bgl II	A^GATCT	E027	1000	O
Bgl II	Bgl II	TCTAG^A	E028	5000
Bis I	Bis I	G(5mC)^NGC	E485	40	*	W
Bis I	Bis I	CGN^(5mC)G	E486	200
Bls I	Bls I	G(5mC)N^GC	E533	100	W
Bls I	Bls I	(5mC)G^N(5mC)G	E534	500
Bme18 I	Ava II	G^GWCC	E029	1000	O	W
Bme18 I	Ava II	CCWG^G	E030	5000
Bmt I	Nhe I	GCTAG^C	E457	1000	W	Y
Bmt I	Nhe I	C^GATCG	E458	5000
Bmu I	Bfi I	ACTGGG(N)5^	E487	100	Y	B,G
Bmu I	Bfi I	TGACCC(N)4^	E488	500
Bpm I	Gsu I	CTGGAG(N)16^	E467	50	W	O
Bpm I	Gsu I	GACCTC(N)14^	E468	250
Bpu10 I	Bpu10 I	CC^TNAGC	E149	200	K
Bpu10 I	Bpu10 I	GGANT^CG	E150	1000
Bpu14 I	Asu II	TT^CGAA	E033	1000	G	Y
Bpu14 I	Asu II	AAGC^TT	E034	5000
Bsa29 I	Cla I	AT^CGAT	E205	1000	G	Y
Bsa29 I	Cla I	TAGC^TA	E206	5000
Bsc4 I	BsiY I	CCNNNNN^NNGG	E219	500	W	B,G
Bsc4 I	BsiY I	GGNN^NNNNNCC	E220	2500

New Enzymes for Your Research

Methyl Specific & Methyl Tolerating Restriction Endonucleases

Selective for (mA, 5mC) Methylation

Cat#

Acronym

DNA Strands

Pack Size units

Alternative Tools for Epigenomic Profiling

Sensitive to dcm or CpG Methylation

Cat #

Acronym

DNA Strands

Pack size units

Insensitive to dcm or CpG Methylation

Cat#

Acronym

DNA Strands

Pack size units

Restriction Endonucleases

Recognition sequence

* means individual buffer; color denotes buffers matching activity range for given set of enzymes. Buffer Components see here

Bse1 I through MroX I

Enzyme

Prototype

Recognition sequence

Cat.No.

Package a.u.

SE Buffer 100% Activ

SE Buffer 75-100% Activ

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DNA Enzymes

DNA METHYLTRANSFERASES

Cat.No

Enzyme

Cat.No

Cat. No

Packageu.a.

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Package
u.a.