Signal Transduction

In Focus: ß-Catenin and AKT

ß-Catenin

ß-Catenin forms part of the cadherin / adhesion complex and presents a focal point in wnt signaling. It has been suggested that ß-catenin may also get involved with activation of the erk signaling pathway, supporting carcinogenesis. Dephospho ß-Catenin represents the active signaling part of the protein while post-translational accumulation has been placed under negative control through phosphorylation. This drives the protein towards ubiquitination and degradation.

Most of our signal transduction antibodies have been raised in, at least, two host animals and have been rigorously tested in Western Blot and Immunhistochemistry. A few are also suitable for immunoprecipitation.

Symansis ß catenin antibody from sheep shows a huge dynamic range in Western Blot. This has been exploited in a signaling array plate which offers flexible combination with antibodies for creb, GSK 3 alpha and beta or other parameters of interest: the Multi Kinase ELISA Array.

Phospho AKT Pathway Sampler Kit

051002	5 x 50 ug
contains 5 individual antibodies for IHC, WB raised against members of the AKT pathway	pdf

Interested in More Pathway Samplers? Inquire

AKT

AKT, also known as protein kinase B, takes a key role in Lipid signaling (specific: Phosphatidyl-3,4,5-trisphosphate PI345P3 and the bisphosphate PI34P2 as substrates), for example in neutrophils. A key element of AKT activation is phosphorylation at thr308. Involvement with other signaling pathways occurs in myocytes, natural killer cells and more. In particular, Symansis offers an antibody directed against a substrate that is a high molecular weight Rab GTPase activator. Antibody 3028 P2 detects this substrate which has been implied in the regulation of GLUT4 translocation. See below for the pdf and for a selection of AKT related antibodies

3028	1 x 100 ug
	Anti-GTPase Activator
E051002	5 x 50 ug
	Anti-Phospho-AKT sampler Kit
E011055	1 x 100 ug
	anti-Phospho-AKT Thr308

Unphosphorylated ß-Catenin

Selective for Dephospho-ß-Catenin

Apoptosis_Sampler

More Pathway Antibodies

Inhibitors, Methyltransferases, Kinases

Kinase Pathway Screen

New Flexible Array Offers Combinatorial Signal Analysis

Assemble your array by selecting enough 2x8 well strips to set up a 96 well microtiter plate (see the menu on the picture below). Choose from 9 pre-assembled sets or focus on PI3 Kinase downstream signalling. A 2 hrs protocol yields semiquantitative data about selected phosphosignaling pathways in your cell lysate. Very economical, yet more sensitive and with a higher dynamic range than standard Western Blot read-outs, this colorimetric assay offers unique combinatorial set-ups, especially upon tandem use with Symansis inhibitors or Inhibitor Arrays.

new 96 well (6 x 16) Kinase Antibody ELISAs, each with specificity for up to 6 different phosphopeptides and active catenin

MKA 001	MKA002	MKA 003	MKA 004	MKA 005
Choose	AKT 1/2	GSK 3 a/ß	Dephospho Catenin	erk 1/2

MKA 006	MKA 007	MKA 008	MKA 010
CREB (pS133)	p38 alpha	GSK/beta Catenin	AKT / GSK

Flexible Kinase Inhibitor Arrays

New Analyze the response of your target cell against inhibitors

using Symansis inhibitor arrays saves time and costs. Optimize inhibitor use by maintaining a concentration window close to the EC50 of your target cell. Current range lists two arrays for screening PI3 Kinase and MAP Kinase family.

As an alternative choose from 150 + highly purified inhibitors with published biological targets and more to come. Ask us to customize arrays for your specific purposes! Quote upon request. See our increasing range of inhibitors here or download a list.

Research Tools PI3 Kinase Family

PI 3 Kinase Inhibitor Array - the first of its kind

Descriptive Video-Sequence Download Flyer

Kinases

Cat.No.	Description	Specificity	[ug]	€
RPK1001	AKT1, active	Human	20	250
RPK1005	AKT1, unactive	Human	20	180
RPK1010	AKT2, active	Human	100	250
RPK1015	AKT2, unactive	Human	20	180
RPK1020	AKT3, active	Human	20	250
RPK1025	AMPKa, inactive	Human	10	250
RPK1030	CHK2, active, E.coli	Human	20	250
RPK1035	CHK2, active, Sf9	Human	20	250
RPK1040	CK2 alpha 1, active	Human	10	150
RPK1045	CK2 alpha 2, active	Human	10	150
RPK1050	CK2 beta	Human	10	150
RPK1060	ERK1, active	Human	10	250
RPK1065	ERK1, inactive	Human	20	150
RPK1070	ERK2, active	Human	5	250
RPK1075	ERK2, inactive	Human	10	150
RPK1080	JNK2alpha2, active	Human	100	250
RPK1085	MEK1, mutant, active	Human	10	250
RPK1090	MKK6, mutant, active	Human	20	250
RPK1095	p38alpha/SAPK2A, active	Human	20	250
RPK1100	p38alpha/SAPK2A,inactive	Human	20	150
RPK1110	PKA alpha, active catalytic	Human	50	250

Methyltransferases

New Methyltransferase-Enzymes and Assays

Lys-Methyl Transferase [G9A]	PRMT 1
PRDM2/RIZ1	Set 2

Top or Home

New Inhibitors

AUR (CDK)

SY-JNJ7706621

JAK2

SY-TG101348

CDK

SY-AT-7519

FMS

SY-Ki20227

MEK

SY-PD0325901

PI 3K beta

SY-TGX221

PI 3K p110alpha

SY-A66
Sun M et al (2010)PNAS 107,15547-52

Multikinase [Motesanib]

SY-AMG706

More DNA Modifying Enzymes ,

Nucleic Acid Extraction and Transfection